Recent papers
2024:
Swiatlowska, P., Tipping, W., Marhuenda, E., Severi, P., Fomin, V., Yang, Z., Xiao, Q., Graham, D., Shanahan, C. & Iskratsch, T. Hypertensive Pressure Mechanosensing Alone Triggers Lipid Droplet Accumulation and Transdifferentiation of Vascular Smooth Muscle Cells to Foam Cells. Advanced Science, e2308686 (2024). Cover Article
2023:
J.A., Arcos-Villacis, N., Battey, E., Boogerd, C., Marhuenda, E., Hodzic, D., Prin, F., Mohun, T., Catibog, N., Tapia, O., Gerace, L., Iskratsch, T., Shah, A.M. & Stroud, M.J. Lem2 is essential for cardiac development by maintaining nuclear integrity. Cardiovasc Res, cvad061, https://doi.org/10.1093/cvr/cvad061(2023).
Battey, E., Ross, J.A., Hoang, A., Wilson, D.G.S., Levy, Y., Pollock, R.D., Kalakoutis, M., Pugh, J.N., Close, G.L., Ellison-Hughes, G.M., Lazarus, N.R., Iskratsch, T., Harridge, S.D.R., Ochala, J. & Stroud, M.J. Exercise induces myonuclear remodelling in humans independently of age. J Physiology https://doi.org/10.1113/JP284128 (2023).
Jacques, R., Zhou, B., Marhuenda, E., Gorecki, J., Das, A., Iskratsch, T. & Krause, S. Photoelectrochemical imaging of action potentials for single cardiomyocytes through contact force manipulation of organoids. Biosensor and Bioelectronics10.1016/j.bios.2022.115024 (2023).
Swiatlowska, P. & Iskratsch, T‡. Cardiovascular mechano-epigenetics: force and gene expression dialogue. Cardiovascular Drugs and Therapy. https://doi.org/10.1007/s10557-022-07422-z (2023)
2022:
Wilson, D.G.S., Tinker, A. & Iskratsch, T‡. The role of the dystrophin glycoprotein complex in muscle cell mechanotransduction. 5, 1022 Commun Biol (2022).
Olson, W., He, R., Benedetto, A., Iskratsch, T., Shaitan, K. & Hall, D. Editors' roundup: October 2022. Biophys Rev 14, 1085-1091 (2022).
Swiatlowska, P., Sit, B., Feng, Z., Marhuenda, E., Xanthis, I., Zingaro, S., Ward, M., Zhou, X., Xiao, Q., Shanahan, C., Jones, G.E., Yu, C.-h. & Iskratsch, T‡. Pressure and stiffness sensing together regulate vascular smooth muscle cell phenotype switching. Science Advances 8, eabm3471 (2022).
Hawkes, W., Marhuenda, E., Reynolds, P., O'Neill, C., Pandey, P., Samuel Wilson, D.G., Freeley, M., Huang, D., Hu, J., Gondarenko, S., Hone, J., Gadegaard, N., Palma, M. & Iskratsch, T‡. Regulation of cardiomyocyte adhesion and mechanosignalling through distinct nanoscale behaviour of integrin ligands mimicking healthy or fibrotic extracellular matrix. Phil Trans R Soc B 377, 20220021 (2022).
Wilson, D.G.S. & Iskratsch, T‡. . The Journal of cell biology 221 (2022).
2021:
Marhuenda, E., Fabre, C., Zhang, C., Martin-Fernandez, M., Iskratsch, T., Saleh, A., Bauchet, L., Cambedouzou, J., Hugnot, J.P., Duffau, H., Dennis, J.W., Cornu, D. & Bakalara, N. Glioma stem cells invasive phenotype at optimal stiffness is driven by MGAT5 dependent mechanosensing. J Exp Clin Cancer Res 40, 139 (2021).
Swiatlowska, P. & Iskratsch, T‡. Cardiovascular mechanobiology—a Special Issue to look at the state of the art and the newest insights into the role of mechanical forces in cardiovascular development, physiology and disease. Biophys Reviews 14 (2021).
Swiatlowska, P. & Iskratsch, T‡. Announcing the call for the Special Issue on "Cardiovascular mechanobiology-a special issue to look at the state of the art and the newest insights into the role of mechanical forces in cardiovascular development, physiology, and disease". Biophys Reviews 13, 307-308 (2021).
Swiatlowska, P. & Iskratsch, T‡. Tools for studying and modulating (cardiac muscle) cell mechanics and mechanosensing across the scales. 13, 611–623 Biophysical Reviews (2021).
2020:
Di Cio, S., Iskratsch, T., Connelly, J.T. & Gautrot, J.E. Contractile myosin rings and cofilin-mediated actin disassembly orchestrate ECM nanotopography sensing. Biomaterials 232, 119683 (2020).
Ward, M. & Iskratsch, T‡. Mix and (mis-)match - The mechanosensing machinery in the changing environment of the developing, healthy adult and diseased heart. Biochim Biophys Acta Mol Cell Res 1867, 118436 (2020).
2019:
Xanthis, I. & Iskratsch, T‡. Lamin-A Mechano-Protects the Heart. Developmental Cell 49, 821-822 (2019).
Hawkes, W., Huang, D., Reynolds, P., Hammond, L., Ward, M., Gadegaard, N., Marshall, J.F., Iskratsch, T‡. & Palma, M. Probing the nanoscale organisation and multivalency of cell surface receptors: DNA origami nanoarrays for cellular studies with single-molecule control. Faraday Discuss 219, 203-219 (2019).
Pedrosa, A.R., Bodrug, N., Gomez-Escudero, J., Carter, E.P., Reynolds, L.E., Georgiou, P.N., Fernandez, I., Lees, D.M., Kostourou, V., Alexopoulou, A.N., Batista, S., Tavora, B., Serrels, B., Parsons, M., Iskratsch, T. & Hodivala-Dilke, K.M. Tumor Angiogenesis Is Differentially Regulated by Phosphorylation of Endothelial Cell Focal Adhesion Kinase Tyrosines-397 and -861. Cancer research 79, 4371-4386 (2019).
Sit, B., Gutmann, D. & Iskratsch, T‡. Costameres, dense plaques and podosomes: the cell matrix adhesions in cardiovascular mechanosensing. J Muscle Res Cell Motil 40, 197-209 (2019).
2018:
Ciuba, K., Hawkes, W., Tojkander, S., Kogan, K., Engel, U., Iskratsch, T. & Lappalainen, P. Calponin-3 is critical for coordinated contractility of actin stress fibers. Scientific Reports 8, 17670 (2018).
Pandey, P., Hawkes, W., Hu, J., Megone, W.V., Gautrot, J., Anilkumar, N., Zhang, M., Hirvonen, L., Cox, S., Ehler, E., Hone, J., Sheetz, M. & Iskratsch, T‡. Cardiomyocytes Sense Matrix Rigidity through a Combination of Muscle and Non-muscle Myosin Contractions. Developmental Cell 44, 326-336 e323 (2018).
other papers:
Lee, N.M., Erisken, C., Iskratsch, T., Sheetz, M., Levine, W.N. & Lu, H.H. Polymer fiber-based models of connective tissue repair and healing. Biomaterials 112, 303-312 (2017).
Meacci, G., Wolfenson, H., Liu, S., Stachowiak, M.R., Iskratsch, T., Mathur, A., Ghassemi, S., Gauthier, N., Tabdanov, E., Lohner, J., Gondarenko, A., Chander, A.C., Roca-Cusachs, P., O'Shaughnessy, B., Hone, J. & Sheetz, M.P. alpha-Actinin links extracellular matrix rigidity-sensing contractile units with periodic cell-edge retractions. Mol Biol Cell 27, 3471-3479 (2016).
Santos, L.C., Blair, D.A., Kumari, S., Cammer, M., Iskratsch, T., Herbin, O., Alexandropoulos, K., Dustin, M.L. & Sheetz, M.P. Actin polymerization-dependent activation of Cas-L promotes immunological synapse stability. Immunol Cell Biol (2016).
Wolfenson, H., Meacci, G., Liu, S., Stachowiak, M.R., Iskratsch, T., Ghassemi, S., Roca-Cusachs, P., O'Shaughnessy, B., Hone, J. & Sheetz, M.P. Tropomyosin controls sarcomere-like contractions for rigidity sensing and suppressing growth on soft matrices. Nature Cell Biol 18, 33-42 (2016).
Tabdanov, E., Gondarenko, S., Kumari, S., Liapis, A., Dustin, M.L., Sheetz, M.P., Kam, L.C. & Iskratsch, T‡. Micropatterning of TCR and LFA-1 ligands reveals complementary effects on cytoskeleton mechanics in T cells. Integr Biol (Camb) 7, 1272-1284 (2015).
Iskratsch, T., Wolfenson, H. & Sheetz, M.P. Appreciating force and shape-the rise of mechanotransduction in cell biology. Nature Rev Mol Cell Biol 15, 825-833 (2014).
Dwyer, J., Pluess, M., Iskratsch, T., Dos Remedios, C.G. & Ehler, E. The formin FHOD1 in cardiomyocytes. Anat Rec (Hoboken) 297, 1560-1570 (2014).
Zhang, X., Moore, S.W., Iskratsch, T. & Sheetz, M.P. N-WASP-directed actin polymerization activates Cas phosphorylation and lamellipodium spreading. J Cell Sci 127, 1394-1405 (2014).
Wolfenson, H., Iskratsch, T*. & Sheetz, M.P. Early events in cell spreading as a model for quantitative analysis of biomechanical events. Biophys J 107, 2508-2514 (2014).Iskratsch, T., Reijntjes, S., Dwyer, J., Toselli, P., Degano, I.R., Dominguez, I. & Ehler, E. Two distinct phosphorylation events govern the function of muscle FHOD3. Cell Mol Life Sci 70, 893-908 (2013).
Iskratsch, T‡., Yu, C.H., Mathur, A., Liu, S., Stevenin, V., Dwyer, J., Hone, J., Ehler, E. & Sheetz, M. FHOD1 is needed for directed forces and adhesion maturation during cell spreading and migration. Developmental Cell 27, 545-559 (2013).
Lynch, C.D., Lazar, A.M., Iskratsch, T., Zhang, X. & Sheetz, M.P. Endoplasmic spreading requires coalescence of vimentin intermediate filaments at force-bearing adhesions. Mol Biol Cell 24, 21-30 (2013).
Roca-Cusachs*, P., Iskratsch, T*. & Sheetz, M.P. Finding the weakest link: exploring integrin-mediated mechanical molecular pathways. J Cell Sci 125, 3025-3038 (2012).
Dwyer, J., Iskratsch, T. & Ehler, E. Actin in striated muscle: recent insights into assembly and maintenance. Biophys Rev 4, 17-25 (2012).
Iskratsch, T. & Ehler, E. Formin-g muscle cytoarchitecture. Bioarchitecture 1, 66-68 (2011).
Iskratsch, T., Lange, S., Dwyer, J., Kho, A.L., dos Remedios, C. & Ehler, E. Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance. J Cell Biol 191, 1159-1172 (2010).
Gutternigg, M., Rendic, D., Voglauer, R., Iskratsch, T. & Wilson, I.B. Mammalian cells contain a second nucleocytoplasmic hexosaminidase. Biochem J 419, 83-90 (2009).
Herbst, R., Iskratsch, T., Unger, E. & Bittner, R.E. Aberrant development of neuromuscular junctions in glycosylation-defective Large(myd) mice. Neuromuscul Disord 19, 366-378 (2009).
Iskratsch, T., Braun, A., Paschinger, K. & Wilson, I.B. Specificity analysis of lectins and antibodies using remodeled glycoproteins. Anal Biochem 386, 133-146 (2009).
‡ Corresponding Author
* Equal contribution